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Celox Laboratories tcm (mouse serum replacement)
Tcm (Mouse Serum Replacement), supplied by Celox Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+serum+replacement/mouse+serum+replacement/pmc04076748-78-12-16
Average 90 stars, based on 1 article reviews
tcm (mouse serum replacement) - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Proliferation Assay:

Article Title: Administration of interleukin-12 prevents mite Der p 1 allergen-IgE antibody production and airway eosinophil infiltration in an animal model of airway inflammation.
Article Snippet: To measure the Der p 1-speci®c T-cell proliferative response, Der p 1-immunized mice were sacri®ced and 3 ́ 105/well spleen cells were cultured with Der p 1 (10 mg/ml) or phytohaemagglutinin (PHA, 10 mg/ml) or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN) plus 2% fetal calf serum (FCS).

Article Title: Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF- κ B Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice
Article Snippet: The peritoneal macrophages were centrifuged and were grown in RPMI-1640 medium (Hyclone, Logan, UT, USA) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN, USA) and antibiotic-antimycotic (Atlanta Biologicals, Norcross, GA, USA).

Article Title: Administration of polysaccharides from Antrodia camphorata modulates dendritic cell function and alleviates allergen-induced T helper type 2 responses in a mouse model of asthma
Article Snippet: Antigen-specific proliferative assay For the proliferation assay, 3 × 10 5 freshly isolated spleen cells/well were cultured with OVA (10, 50 and 100 μg/ml) and 10 μg/ml phytohaemagglutinin (PHA, Sigma), or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement; Celox, Hopkins, MN) plus 2% fetal bovine serum.

Article Title: Treatment of murine lupus using nucleosomal T cell epitopes identified by bone marrow-derived dendritic cells.
Article Snippet: Objective.. Systemic lupus erythematosus (SLE) is characterized by the existence of a heterogeneous group of autoantibodies directed against intact nuclear structures, such as nucleosomes.. The most prominent of these autoantibodies are those directed against doublestranded DNA (dsDNA) and histones.

Article Title: Tamoxifen alleviates disease severity and decreases double negative T cells in autoimmune MRL-lpr/lpr mice
Article Snippet: For cell proliferation assay, the cells were cultured in serum-free medium (AIM-V, Gibco/BRL, Gaithersburg, MD) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN).

Article Title: Opposite effects of low and high dose supplementation of vitamin E on survival of MRL/lpr mice.
Article Snippet: Spleen cells at a concentration of 5 06 cells/mL were placed in 24-well plates in Roswell Park emorial Institute (RPMI)-1640 medium supplemented ith 10% TCM (mouse serum replacement, Celox Corp., opkins, MN).

Isolation:

Article Title: Administration of interleukin-12 prevents mite Der p 1 allergen-IgE antibody production and airway eosinophil infiltration in an animal model of airway inflammation.
Article Snippet: To measure the Der p 1-speci®c T-cell proliferative response, Der p 1-immunized mice were sacri®ced and 3 ́ 105/well spleen cells were cultured with Der p 1 (10 mg/ml) or phytohaemagglutinin (PHA, 10 mg/ml) or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN) plus 2% fetal calf serum (FCS).

Article Title: Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF- κ B Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice
Article Snippet: The peritoneal macrophages were centrifuged and were grown in RPMI-1640 medium (Hyclone, Logan, UT, USA) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN, USA) and antibiotic-antimycotic (Atlanta Biologicals, Norcross, GA, USA).

Article Title: Administration of polysaccharides from Antrodia camphorata modulates dendritic cell function and alleviates allergen-induced T helper type 2 responses in a mouse model of asthma
Article Snippet: Antigen-specific proliferative assay For the proliferation assay, 3 × 10 5 freshly isolated spleen cells/well were cultured with OVA (10, 50 and 100 μg/ml) and 10 μg/ml phytohaemagglutinin (PHA, Sigma), or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement; Celox, Hopkins, MN) plus 2% fetal bovine serum.

Article Title: Treatment of murine lupus using nucleosomal T cell epitopes identified by bone marrow-derived dendritic cells.
Article Snippet: Objective.. Systemic lupus erythematosus (SLE) is characterized by the existence of a heterogeneous group of autoantibodies directed against intact nuclear structures, such as nucleosomes.. The most prominent of these autoantibodies are those directed against doublestranded DNA (dsDNA) and histones.

Article Title: Tamoxifen alleviates disease severity and decreases double negative T cells in autoimmune MRL-lpr/lpr mice
Article Snippet: For cell proliferation assay, the cells were cultured in serum-free medium (AIM-V, Gibco/BRL, Gaithersburg, MD) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN).

Article Title: Opposite effects of low and high dose supplementation of vitamin E on survival of MRL/lpr mice.
Article Snippet: Spleen cells at a concentration of 5 06 cells/mL were placed in 24-well plates in Roswell Park emorial Institute (RPMI)-1640 medium supplemented ith 10% TCM (mouse serum replacement, Celox Corp., opkins, MN).

Cell Culture:

Article Title: Administration of interleukin-12 prevents mite Der p 1 allergen-IgE antibody production and airway eosinophil infiltration in an animal model of airway inflammation.
Article Snippet: To measure the Der p 1-speci®c T-cell proliferative response, Der p 1-immunized mice were sacri®ced and 3 ́ 105/well spleen cells were cultured with Der p 1 (10 mg/ml) or phytohaemagglutinin (PHA, 10 mg/ml) or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN) plus 2% fetal calf serum (FCS).

Article Title: Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF- κ B Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice
Article Snippet: The peritoneal macrophages were centrifuged and were grown in RPMI-1640 medium (Hyclone, Logan, UT, USA) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN, USA) and antibiotic-antimycotic (Atlanta Biologicals, Norcross, GA, USA).

Article Title: Administration of polysaccharides from Antrodia camphorata modulates dendritic cell function and alleviates allergen-induced T helper type 2 responses in a mouse model of asthma
Article Snippet: Antigen-specific proliferative assay For the proliferation assay, 3 × 10 5 freshly isolated spleen cells/well were cultured with OVA (10, 50 and 100 μg/ml) and 10 μg/ml phytohaemagglutinin (PHA, Sigma), or medium only in RPMI-1640 medium containing 2% TCM (mouse serum replacement; Celox, Hopkins, MN) plus 2% fetal bovine serum.

Article Title: Treatment of murine lupus using nucleosomal T cell epitopes identified by bone marrow-derived dendritic cells.
Article Snippet: Objective.. Systemic lupus erythematosus (SLE) is characterized by the existence of a heterogeneous group of autoantibodies directed against intact nuclear structures, such as nucleosomes.. The most prominent of these autoantibodies are those directed against doublestranded DNA (dsDNA) and histones.

Article Title: Tamoxifen alleviates disease severity and decreases double negative T cells in autoimmune MRL-lpr/lpr mice
Article Snippet: For cell proliferation assay, the cells were cultured in serum-free medium (AIM-V, Gibco/BRL, Gaithersburg, MD) supplemented with 2% TCM (mouse serum replacement, Celox Corp., Hopkins, MN).

Article Title: Opposite effects of low and high dose supplementation of vitamin E on survival of MRL/lpr mice.
Article Snippet: Spleen cells at a concentration of 5 06 cells/mL were placed in 24-well plates in Roswell Park emorial Institute (RPMI)-1640 medium supplemented ith 10% TCM (mouse serum replacement, Celox Corp., opkins, MN).



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